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TBC1D4 Polyclonal antibody

TBC1D4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 20024-1-AP

Print datasheet

Synonyms

TBC1 domain family, member 4, TBC1 domain family member 4, KIAA0603, AS160, Akt substrate of 160 kDa

验证数据展示

  • WB analysis using 20024-1-AP

    WB analysis using 20024-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20024-1-AP (TBC1D4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 20024-1-AP

    IHC staining of mouse colon using 20024-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 20024-1-AP

    IHC staining of mouse colon using 20024-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat skeletal muscle using 20024-1-AP

    IHC staining of rat skeletal muscle using 20024-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 20024-1-AP

    IHC staining of mouse skeletal muscle using 20024-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 20024-1-AP

    IF Staining of HeLa using 20024-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TBC1D4 antibody (20024-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HeLa using 20024-1-AP

    FC experiment of HeLa using 20024-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TBC1D4 (20024-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of Jurkat using 20024-1-AP

    FC experiment of Jurkat using 20024-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human TBC1D4 (20024-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse colon tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20024-1-AP targets TBC1D4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TBC1 domain family, member 4
Synonyms TBC1 domain family, member 4, TBC1 domain family member 4, KIAA0603, AS160, Akt substrate of 160 kDa
Calculated Molecular Weight 147 kDa
Observed Molecular Weight 160 kDa
GenBank Accession NumberNM_014832
Gene Symbol TBC1D4
Gene ID (NCBI) 9882
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60343
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBC1D4, also named as AS160, KIAA0603, may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. The antibody recognizes all the isoform of TBC1D4.

实验方案

Product Specific Protocols
WB protocol for TBC1D4 antibody 20024-1-APDownload protocol
IHC protocol for TBC1D4 antibody 20024-1-APDownload protocol
IF protocol for TBC1D4 antibody 20024-1-APDownload protocol
FC protocol for TBC1D4 antibody 20024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols