• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TBC1D4 Polyclonal antibody

TBC1D4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24667-1-AP

Print datasheet

Synonyms

TBC1 domain family, member 4, TBC1 domain family member 4, KIAA0603, AS160, Akt substrate of 160 kDa

验证数据展示

  • WB analysis using 24667-1-AP

    WB analysis using 24667-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24667-1-AP (TBC1D4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 24667-1-AP

    WB analysis using 24667-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24667-1-AP (TBC1D4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 24667-1-AP

    IHC staining of human colon using 24667-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50.

  • IHC staining of human colon using 24667-1-AP

    IHC staining of human colon using 24667-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50.

  • IHC staining of mouse colon using 24667-1-AP

    IHC staining of mouse colon using 24667-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24667-1-AP (TBC1D4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 24667-1-AP

    IHC staining of mouse colon using 24667-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24667-1-AP (TBC1D4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 24667-1-AP

    IF Staining of HeLa using 24667-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TBC1D4 antibody (24667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHCT 116 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells
Positive IHC detected inmouse colon tissue, human colon tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24667-1-AP targets TBC1D4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, rat
Immunogen TBC1D4 fusion protein Ag20327 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TBC1 domain family, member 4
Synonyms TBC1 domain family, member 4, TBC1 domain family member 4, KIAA0603, AS160, Akt substrate of 160 kDa
Calculated Molecular Weight 1299 aa, 147 kDa
Observed Molecular Weight 160 kDa
GenBank Accession NumberBC151239
Gene Symbol TBC1D4
Gene ID (NCBI) 9882
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60343
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBC1D4, also named as AS160, KIAA0603, may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. The antibody recognizes all the isoform of TBC1D4.

实验方案

Product Specific Protocols
WB protocol for TBC1D4 antibody 24667-1-APDownload protocol
IHC protocol for TBC1D4 antibody 24667-1-APDownload protocol
IF protocol for TBC1D4 antibody 24667-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols