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验证数据展示

  • WB analysis using 82911-7-RR

    WB analysis using 82911-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82911-7-RR (TBCD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 82911-7-RR

    IF Staining of HepG2 using 82911-7-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TBCD antibody (82911-7-RR, Clone: 230209F3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of MCF-7 using 82911-7-RR

    FC experiment of MCF-7 using 82911-7-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug TBCD Recombinant antibody (82911-7-RR, Clone:230209F3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82911-7-RR

    Affinity and Kinetic Characterization of 82911-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82911-7-RR against Human TBCD were performed. The affinity constant is 97.6 pM.

TBCD Recombinant antibody

TBCD Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 82911-7-RR

产品说明书

CloneNo. 230209F3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

SSD-1, SSD1, PP1096, KIAA0988, Beta-tubulin cofactor D

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82911-7-RR targets TBCD in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 TBCD fusion protein Ag6310 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tubulin folding cofactor D
别名 SSD-1, SSD1, PP1096, KIAA0988, Beta-tubulin cofactor D
计算分子量 133 kDa
观测分子量 130 kDa
GenBank蛋白编号BC003094
基因名称 TBCD
Gene ID (NCBI) 6904
RRIDAB_3670643
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BTW9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TBCD antibody 82911-7-RRDownload protocol
IF protocol for TBCD antibody 82911-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols