验证数据展示
经过测试的应用
| Positive WB detected in | mouse pancreas tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13540-1-AP targets TBL1X in WB, IP, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IP, CoIP, ChIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | TBL1X fusion protein Ag4477 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | transducin (beta)-like 1X-linked |
| Synonyms | EBI, SMAP55, TBL1, TBL1X |
| Calculated Molecular Weight | 526 aa, 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC032708 |
| Gene Symbol | TBL1X |
| Gene ID (NCBI) | 6907 |
| RRID | AB_2199783 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60907 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TBL1X is an essential subunit of the nuclear receptor corepressor (NCoR)-silencing mediator for retinoid and thyroid hormone receptors (SMRT) complex, the major TH receptor (TR) corepressor (CoR) involved in T3-regulated gene expression. TBL1X defects were recently associated with central hypothyroidism and hearing loss. (PMID: 30591955, PMID: 27603907)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TBL1X antibody 13540-1-AP | Download protocol |
| IHC protocol for TBL1X antibody 13540-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Development Setd5 is essential for mammalian development and the co-transcriptional regulation of histone acetylation. | ||
Int J Biol Sci TBL1X and Flot2 form a positive feedback loop to promote metastasis in nasopharyngeal carcinoma.
| ||
Cell Chem Biol HDAC3 and HDAC8 PROTAC dual degrader reveals roles of histone acetylation in gene regulation |
