验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HeLa cells, human placenta tissue, Jurkat cells, mouse pancreas tissue, mouse placenta tissue |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human pancreas cancer tissue, human heart tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12488-1-AP targets TBL2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | TBL2 fusion protein Ag3200 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transducin (beta)-like 2 |
| 别名 | Williams-Beuren syndrome chromosomal region 13 protein, WBSCR13, UNQ563/PRO1125, Transducin beta-like protein 2, Transducin beta like protein 2 |
| 计算分子量 | 446 aa, 50 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC012938 |
| 基因名称 | TBL2 |
| Gene ID (NCBI) | 26608 |
| RRID | AB_2199923 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y4P3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TBL2 belongs to a member of the beta-transducin protein family. This gene is commonly deleted in Williams-Beuren syndrome, a developmental disorder with multi-system manifestations caused by haploinsufficiency for contiguous genes deleted in chromosome region 7q11.23. TBL2 encodes a protein with four putative WD40 repeats, which may be involved in some intracellular signaling pathway.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TBL2 antibody 12488-1-AP | Download protocol |
| IHC protocol for TBL2 antibody 12488-1-AP | Download protocol |
| IF protocol for TBL2 antibody 12488-1-AP | Download protocol |
| IP protocol for TBL2 antibody 12488-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) TBL2 Promotes Tumorigenesis via PRMT5/WDR77-Mediated AKT Activation in Breast Cancer | ||
PLoS One TBL2 Is a Novel PERK-Binding Protein that Modulates Stress-Signaling and Cell Survival during Endoplasmic Reticulum Stress.
| ||
J Cell Biochem TBL2 Associates With ATF4 mRNA Via Its WD40 Domain and Regulates Its Translation During ER Stress. | ||
Biochem Biophys Res Commun The endoplasmic reticulum-localized protein TBL2 interacts with the 60S ribosomal subunit. | ||
Ann Surg Oncol Transducin Beta-Like 2 is a Potential Driver Gene that Adapts to Endoplasmic Reticulum Stress to Promote Tumor Growth of Lung Adenocarcinoma
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