验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, COLO 320 cells, Jurkat cells |
Positive IHC detected in | human liver tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IHC | See 1 publications below |
产品信息
66307-1-Ig targets TBL2 in WB, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | TBL2 fusion protein Ag24225 种属同源性预测 |
宿主/亚型 | Mouse / IgA |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | transducin (beta)-like 2 |
别名 | TBL2, transducin (beta) like 2, Transducin beta like protein 2, WBSCR13, WS betaTRP |
计算分子量 | 446 aa, 50 kDa |
观测分子量 | 50 kDa |
GenBank蛋白编号 | BC012938 |
基因名称 | TBL2 |
Gene ID (NCBI) | 26608 |
RRID | AB_2881689 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Thiophilic affinity chromatograph |
UNIPROT ID | Q9Y4P3 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TBL2 belongs to a member of the beta-transducin protein family. This gene is commonly deleted in Williams-Beuren syndrome, a developmental disorder with multi-system manifestations caused by haploinsufficiency for contiguous genes deleted in chromosome region 7q11.23. TBL2 encodes a protein with four putative WD40 repeats, which may be involved in some intracellular signaling pathway.
实验方案
Product Specific Protocols | |
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WB protocol for TBL2 antibody 66307-1-Ig | Download protocol |
IHC protocol for TBL2 antibody 66307-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |