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验证数据展示

  • WB analysis using 66564-1-Ig (same clone as 66564-1-PBS)

    WB analysis using 66564-1-Ig (same clone as 66564-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66564-1-Ig (TBR1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    IHC staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66564-1-Ig (TBR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TBR1 antibody included
  • IHC staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    IHC staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66564-1-Ig (TBR1 antibody) at dilution of 1:200 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TBR1 antibody included
  • WB analysis of pig cerebellum using 66564-1-Ig (same clone as 66564-1-PBS)

    WB analysis of pig cerebellum using 66564-1-Ig (same clone as 66564-1-PBS)

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66564-1-Ig (TBR1 antibody) at dilution of 1:2000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    IF Staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TBR1 antibody (66564-1-Ig, Clone: 5C3A5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    IF Staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TBR1 antibody (66564-1-Ig, Clone: 5C3A5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

TBR1 Monoclonal antibody, PBS Only

TBR1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66564-1-PBS

产品说明书

CloneNo. 5C3A5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, ELISA

别名

TBR-1, TBR 1, T-box brain protein 1, T brain 1, T box, brain, 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TBR1 Ready-To-Use IHC Kit. TBR1 primary antibody included.

产品信息

66564-1-PBS targets TBR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 TBR1 fusion protein Ag15262 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 T-box, brain, 1
别名 TBR-1, TBR 1, T-box brain protein 1, T brain 1, T box, brain, 1
计算分子量 682 aa, 74 kDa
观测分子量 74 kDa
GenBank蛋白编号BC104844
基因名称 TBR1
Gene ID (NCBI) 10716
RRIDAB_2881925
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ16650
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TBR1, also named T-box brain protein 1, is a 682 amino acid protein, which contains one T-box DNA-binding domain and localizes in the nucleus. TBR1 is expressed in the brain and as a transcriptional regulator is involved in developmental processes. TBR1 is required for normal brain development.