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TBR1 Monoclonal antibody, PBS Only

TBR1 Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, pig

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

5C3A5

Cat No : 66564-1-PBS

Print datasheet

Synonyms

T box brain protein 1, T box, brain, 1, T brain 1, TBR 1, TBR1, TES 56

验证数据展示

  • WB analysis using 66564-1-Ig (same clone as 66564-1-PBS)

    WB analysis using 66564-1-Ig (same clone as 66564-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66564-1-Ig (TBR1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    IHC staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66564-1-Ig (TBR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TBR1 antibody included
  • IHC staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    IHC staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66564-1-Ig (TBR1 antibody) at dilution of 1:200 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TBR1 antibody included
  • IF Staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    IF Staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66564-1-Ig (TBR1 antibody) at dilution of 1:100 and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    IF Staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66564-1-Ig (TBR1 antibody) at dilution of 1:100 and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

  • WB analysis of pig cerebellum using 66564-1-Ig (same clone as 66564-1-PBS)

    WB analysis of pig cerebellum using 66564-1-Ig (same clone as 66564-1-PBS)

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66564-1-Ig (TBR1 antibody) at dilution of 1:2000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TBR1 Ready-To-Use IHC Kit. TBR1 primary antibody included.

产品信息

66564-1-PBS targets TBR1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, rat, mouse, pig samples.

Tested Applications WB,IHC,IF,ELISA Application Description
Tested Reactivity Human, rat, mouse, pig
Immunogen TBR1 fusion protein Ag15262 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name T-box, brain, 1
Synonyms T box brain protein 1, T box, brain, 1, T brain 1, TBR 1, TBR1, TES 56
Calculated Molecular Weight 682 aa, 74 kDa
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC104844
Gene Symbol TBR1
Gene ID (NCBI) 10716
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16650
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

TBR1, also named as T-box brain protein 1, is a 682 amino acid protein, which contains one T-box DNA-binding domain and localizes in the nucleus. TBR1 is expressed in the brain and as a transcriptional regulator is involved in developmental processes. TBR1 is required for normal brain development.