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验证数据展示

  • WB analysis of A431 using 10177-2-AP

    WB analysis of A431 using 10177-2-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10177-2-AP (TBRG4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 10177-2-AP

    IHC staining of human ovary cancer using 10177-2-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10177-2-AP (TBRG4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 10177-2-AP

    IHC staining of human stomach cancer using 10177-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10177-2-AP (TBRG4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 10177-2-AP

    IHC staining of human stomach cancer using 10177-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10177-2-AP (TBRG4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 10177-2-AP

    IF Staining of HepG2 using 10177-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TBRG4 antibody (10177-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

TBRG4 Polyclonal antibody

TBRG4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10177-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Protein TBRG4, KIAA0948, FASTKD4, FAST kinase domain-containing protein 4, CPR2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HepG2 cells
Positive IHC detected inhuman stomach cancer tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10177-2-AP targets TBRG4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 TBRG4 fusion protein Ag0212 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transforming growth factor beta regulator 4
别名 Protein TBRG4, KIAA0948, FASTKD4, FAST kinase domain-containing protein 4, CPR2
计算分子量 71 kDa
观测分子量 58 kDa
GenBank蛋白编号BC002732
基因名称 TBRG4
Gene ID (NCBI) 9238
RRIDAB_2255699
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ969Z0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TBRG4 antibody 10177-2-APDownload protocol
IHC protocol for TBRG4 antibody 10177-2-APDownload protocol
IF protocol for TBRG4 antibody 10177-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols