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TBX20 Recombinant antibody

TBX20 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240385H6

Cat no : 83414-3-RR

Print datasheet

Synonyms

TBX 20, T-box transcription factor TBX20, T-box protein 20, T box 20, ASD4

验证数据展示

  • IF Staining of H9C2 using 83414-3-RR

    IF Staining of H9C2 using 83414-3-RR

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using TBX20 antibody (83414-3-RR, Clone: 240385H6 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • FC experiment of K562 using 83414-3-RR

    FC experiment of K562 using 83414-3-RR

    1x10^6 K562 were intracellularly stained with 0.25 ug Tbx20 Recombinant Antibody (83414-3-RR, Clone:240385H6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IHC staining of mouse heart using 83414-3-RR

    IHC staining of mouse heart using 83414-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83414-3-RR (TBX20 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83414-3-RR

    Affinity and Kinetic Characterization of 83414-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83414-3-RR against Human TBX20 were performed. The affinity constant is 17 nM.

经过测试的应用

Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inH9C2 cells
Positive FC (Intra) detected inK562

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83414-3-RR is TBX20 Fusion Protein expressed in E. coli.

Tested Applications IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen TBX20 fusion protein Ag18501 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name T-box 20
Synonyms TBX 20, T-box transcription factor TBX20, T-box protein 20, T box 20, ASD4
Calculated Molecular Weight 297 aa, 33 kDa
GenBank Accession NumberBC120946
Gene Symbol TBX20
Gene ID (NCBI) 57057
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UMR3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tbx20 is a transcription factor that is essential for proper heart development in a growing fetus. Any mutations in this gene can result in various forms of congenital heart disease.

实验方案

Product Specific Protocols
IHC protocol for TBX20 antibody 83414-3-RRDownload protocol
IF protocol for TBX20 antibody 83414-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols