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TBX20 Recombinant antibody, PBS Only

TBX20 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240385C12

Cat no : 83414-5-PBS

Print datasheet

Synonyms

TBX 20, T-box transcription factor TBX20, T-box protein 20, T box 20, ASD4

验证数据展示

  • WB analysis using 83414-5-RR (same clone as 83414-5-PBS)

    WB analysis using 83414-5-RR (same clone as 83414-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83414-5-RR (TBX20 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83414-5-PBS in a different storage buffer formulation.

  • FC experiment of k562 using 83414-5-RR (same clone as 83414-5-PBS)

    FC experiment of k562 using 83414-5-RR (same clone as 83414-5-PBS)

    1x10^6 k562 were intracellularly stained with 0.25 ug Tbx20 Recombinant Antibody (83414-5-RR, Clone:240385C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83414-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83414-5-RR

    Affinity and Kinetic Characterization of 83414-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83414-5-RR against Human TBX20 were performed. The affinity constant is 30 nM.

产品信息

83414-5-PBS targets TBX20 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TBX20 fusion protein Ag18501 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name T-box 20
Synonyms TBX 20, T-box transcription factor TBX20, T-box protein 20, T box 20, ASD4
Calculated Molecular Weight 297 aa, 33 kDa
Observed Molecular Weight50-60 kDa
GenBank Accession NumberBC120946
Gene Symbol TBX20
Gene ID (NCBI) 57057
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UMR3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tbx20 is a transcription factor that is essential for proper heart development in a growing fetus. Any mutations in this gene can result in various forms of congenital heart disease.