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TBX20 Recombinant antibody, PBS Only

TBX20 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240385C12

Cat no : 83414-5-PBS

Print datasheet

Synonyms

TBX 20, T-box transcription factor TBX20, T-box protein 20, T box 20, ASD4

验证数据展示

  • WB analysis using 83414-5-RR (same clone as 83414-5-PBS)

    WB analysis using 83414-5-RR (same clone as 83414-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83414-5-RR (TBX20 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83414-5-PBS in a different storage buffer formulation.

  • FC experiment of k562 using 83414-5-RR (same clone as 83414-5-PBS)

    FC experiment of k562 using 83414-5-RR (same clone as 83414-5-PBS)

    1x10^6 k562 were intracellularly stained with 0.25 ug Tbx20 Recombinant Antibody (83414-5-RR, Clone:240385C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83414-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83414-5-RR

    Affinity and Kinetic Characterization of 83414-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83414-5-RR against Human TBX20 were performed. The affinity constant is 30 nM.

产品信息

The immunogen of 83414-5-PBS is TBX20 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TBX20 fusion protein Ag18501 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name T-box 20
Synonyms TBX 20, T-box transcription factor TBX20, T-box protein 20, T box 20, ASD4
Calculated Molecular Weight 297 aa, 33 kDa
Observed Molecular Weight50-60 kDa
GenBank Accession NumberBC120946
Gene Symbol TBX20
Gene ID (NCBI) 57057
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UMR3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tbx20 is a transcription factor that is essential for proper heart development in a growing fetus. Any mutations in this gene can result in various forms of congenital heart disease.