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验证数据展示

  • WB analysis of HeLa using 12447-1-AP

    WB analysis of HeLa using 12447-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of human placenta using 12447-1-AP

    IP experiment of human placenta using 12447-1-AP

    IP result of anti-TBX6 (IP:12447-1-AP, 4ug; Detection:12447-1-AP 1:500) with human placenta tissue lysate 2800ug.

TBX6 Polyclonal antibody

TBX6 Polyclonal Antibody for WB, IP, ELISA
Cat No. 12447-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

DFNB67, T box 6, T box protein 6, TBX6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A375 cells, A549 cells, human lung tissue, human placenta tissue, mouse lung tissue, Raji cells
Positive IP detected inhuman placenta tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12447-1-AP targets TBX6 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 TBX6 fusion protein Ag3050 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 T-box 6
别名 DFNB67, T box 6, T box protein 6, TBX6
计算分子量 436 aa, 47 kDa
观测分子量 58 kDa
GenBank蛋白编号BC026031
基因名称 TBX6
Gene ID (NCBI) 6911
RRIDAB_2200836
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95947
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBX6, also named as T-box transcription factor TBX6, is a 436 amino acid protein, which localizes in the nucleus and forms a dimeric complex with DNA. T-box transcription factor that plays an essential role in the determination of the fate of axial stem cells: neural vs mesodermal. The calculated molecular weight of TBX6 is 47 kDa, but the modified TBX6 is about 58 kDa.

实验方案

Product Specific Protocols
WB protocol for TBX6 antibody 12447-1-APDownload protocol
IP protocol for TBX6 antibody 12447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols