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验证数据展示

  • WB analysis of HeLa using 12447-1-AP

    WB analysis of HeLa using 12447-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of human placenta using 12447-1-AP

    IP experiment of human placenta using 12447-1-AP

    IP result of anti-TBX6 (IP:12447-1-AP, 4ug; Detection:12447-1-AP 1:500) with human placenta tissue lysate 2800ug.

TBX6 Polyclonal antibody

TBX6 Polyclonal Antibody for WB, IP, ELISA
Cat No. 12447-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

DFNB67, T box 6, T box protein 6, TBX6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A375 cells, A549 cells, human lung tissue, human placenta tissue, mouse lung tissue, Raji cells
Positive IP detected inhuman placenta tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12447-1-AP targets TBX6 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen TBX6 fusion protein Ag3050 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name T-box 6
Synonyms DFNB67, T box 6, T box protein 6, TBX6
Calculated Molecular Weight 436 aa, 47 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC026031
Gene Symbol TBX6
Gene ID (NCBI) 6911
RRIDAB_2200836
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95947
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBX6, also named as T-box transcription factor TBX6, is a 436 amino acid protein, which localizes in the nucleus and forms a dimeric complex with DNA. T-box transcription factor that plays an essential role in the determination of the fate of axial stem cells: neural vs mesodermal. The calculated molecular weight of TBX6 is 47 kDa, but the modified TBX6 is about 58 kDa.

实验方案

Product Specific Protocols
WB protocol for TBX6 antibody 12447-1-APDownload protocol
IP protocol for TBX6 antibody 12447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols