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验证数据展示

  • WB analysis using 30215-1-AP

    WB analysis using 30215-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30215-1-AP (TBXAS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 30215-1-AP

    IHC staining of human ovary cancer using 30215-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30215-1-AP (TBXAS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 30215-1-AP

    IHC staining of human ovary cancer using 30215-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30215-1-AP (TBXAS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TBXAS1 Polyclonal antibody

TBXAS1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30215-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

THAS, Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, CYP5A1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, THP-1 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30215-1-AP targets TBXAS1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen TBXAS1 fusion protein Ag30950 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thromboxane A synthase 1 (platelet)
Synonyms THAS, Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, CYP5A1
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC014117
Gene Symbol TBXAS1
Gene ID (NCBI) 6916
RRIDAB_3086266
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24557
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBXAS1 catalyzes the conversion of the prostaglandin endoperoxide into thromboxane A2 as a potent vasoconstrictor and inducer of platelet aggregation. It was identified as a novel airway fibroblast-specific marker with integrin-8 (ITGA8) (PMID:20435685). It was observed in the epithelium. It is a monomer with a molecular weight of 60 kDa. It also has two variants with 60.5 kDa and 52.3 kDa (PMID:1730669).

实验方案

Product Specific Protocols
WB protocol for TBXAS1 antibody 30215-1-APDownload protocol
IHC protocol for TBXAS1 antibody 30215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols