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验证数据展示

  • WB analysis using 30215-1-AP

    WB analysis using 30215-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30215-1-AP (TBXAS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 30215-1-AP

    IHC staining of human ovary cancer using 30215-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30215-1-AP (TBXAS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 30215-1-AP

    IHC staining of human ovary cancer using 30215-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30215-1-AP (TBXAS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TBXAS1 Polyclonal antibody

TBXAS1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30215-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

THAS, Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, CYP5A1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, THP-1 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30215-1-AP targets TBXAS1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 TBXAS1 fusion protein Ag30950 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thromboxane A synthase 1 (platelet)
别名 THAS, Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, CYP5A1
计算分子量 60 kDa
观测分子量 52 kDa
GenBank蛋白编号BC014117
基因名称 TBXAS1
Gene ID (NCBI) 6916
RRIDAB_3086266
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP24557
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBXAS1 catalyzes the conversion of the prostaglandin endoperoxide into thromboxane A2 as a potent vasoconstrictor and inducer of platelet aggregation. It was identified as a novel airway fibroblast-specific marker with integrin-8 (ITGA8) (PMID:20435685). It was observed in the epithelium. It is a monomer with a molecular weight of 60 kDa. It also has two variants with 60.5 kDa and 52.3 kDa (PMID:1730669).

实验方案

Product Specific Protocols
WB protocol for TBXAS1 antibody 30215-1-APDownload protocol
IHC protocol for TBXAS1 antibody 30215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols