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TBXAS1 Polyclonal antibody

TBXAS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30215-1-AP

Print datasheet

Synonyms

THAS, Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, CYP5A1

验证数据展示

  • WB analysis using 30215-1-AP

    WB analysis using 30215-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30215-1-AP (TBXAS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 30215-1-AP

    IHC staining of human ovary cancer using 30215-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30215-1-AP (TBXAS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 30215-1-AP

    IHC staining of human ovary cancer using 30215-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30215-1-AP (TBXAS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inPC-3 cells, THP-1 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30215-1-AP targets TBXAS1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen TBXAS1 fusion protein Ag30950 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thromboxane A synthase 1 (platelet)
Synonyms THAS, Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, CYP5A1
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC014117
Gene Symbol TBXAS1
Gene ID (NCBI) 6916
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24557
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBXAS1 catalyzes the conversion of the prostaglandin endoperoxide into thromboxane A2 as a potent vasoconstrictor and inducer of platelet aggregation. It was identified as a novel airway fibroblast-specific marker with integrin-8 (ITGA8) (PMID:20435685). It was observed in the epithelium. It is a monomer with a molecular weight of 60 kDa. It also has two variants with 60.5 kDa and 52.3 kDa (PMID:1730669).

实验方案

Product Specific Protocols
WB protocol for TBXAS1 antibody 30215-1-APDownload protocol
IHC protocol for TBXAS1 antibody 30215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols