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  • Featured Product
  • KD/KO Validated

TCEB3 Polyclonal antibody

TCEB3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10502-1-AP

Print datasheet

Synonyms

SIII, MSTP059, Elongin-A, Elongin A, Elongin 110 kDa subunit

验证数据展示

  • WB analysis of mouse liver using 10502-1-AP

    WB analysis of mouse liver using 10502-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10502-1-AP (TCEB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10502-1-AP

    WB analysis using 10502-1-AP

    Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 10502-1-AP (TCEB3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 10502-1-AP

    IF Staining of HeLa using 10502-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TCEB3 antibody (10502-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inmouse liver tissue, mouse brain tissue, Jurkat cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ELOA Ready-To-Use IHC Kit. ELOA primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10502-1-AP targets TCEB3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TCEB3 fusion protein Ag0778 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A)
Synonyms SIII, MSTP059, Elongin-A, Elongin A, Elongin 110 kDa subunit
Calculated Molecular Weight 110 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC002883
Gene Symbol TCEB3
Gene ID (NCBI) 6924
RRIDAB_2201278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14241
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TCEB3 antibody 10502-1-APDownload protocol
IF protocol for TCEB3 antibody 10502-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

FASEB J

TCEB3 initiates ovarian cancer apoptosis by mediating ubiquitination and degradation of MCL-1

Authors - Ying Cai
  • KD Validated
View Article