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验证数据展示

  • WB analysis using 32030-1-AP

    WB analysis using 32030-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32030-1-AP (TCF20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

TCF20 Polyclonal antibody

TCF20 Polyclonal Antibody for WB, ELISA
Cat No. 32030-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Stromelysin-1 PDGF-responsive element-binding protein, SPRE-binding protein, SPBP, Protein AR1, Nuclear factor SPBP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, U-251 cells, U2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32030-1-AP targets TCF20 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 TCF20 fusion protein Ag36912 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transcription factor 20 (AR1)
别名 Stromelysin-1 PDGF-responsive element-binding protein, SPRE-binding protein, SPBP, Protein AR1, Nuclear factor SPBP
计算分子量1960aa, 212 kDa
观测分子量250 kDa
GenBank蛋白编号NM_005650
基因名称 TCF20
Gene ID (NCBI) 6942
RRIDAB_3670174
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9UGU0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transcription Factor 20 (TCF20) is a transcriptional activator that binds to the regulatory region of MMP3 and thereby controls stromelysin expression. It stimulates the activity of various transcriptional activators such as JUN, SP1, PAX6 and ETS1, suggesting a function as a coactivator (PMID: 10995766).

实验方案

Product Specific Protocols
WB protocol for TCF20 antibody 32030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols