验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, NCI-H1299 cells, PC-3 cells, Jurkat cells, K-562 cells, THP-1 cells, A549 cells |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 2 publications below |
WB | See 3 publications below |
IHC | See 1 publications below |
产品信息
67140-1-Ig targets TCF3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | Human |
文献引用反应性 | human |
免疫原 | TCF3 fusion protein Ag15734 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) |
别名 | bHLHb21, E2A, ITF1, Kappa E2 binding factor, TCF 3, TCF3, Transcription factor 3, Transcription factor E2 alpha, Transcription factor ITF 1 |
计算分子量 | 654 aa, 68 kDa |
观测分子量 | 68-70 kDa |
GenBank蛋白编号 | BC110580 |
基因名称 | TCF3 |
Gene ID (NCBI) | 6929 |
RRID | AB_2882439 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P15923 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for TCF3 antibody 67140-1-Ig | Download protocol |
IF protocol for TCF3 antibody 67140-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Reproduction TCF3 regulates human endometrial stromal cell proliferation and migration in RPL.
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Mol Med Rep Expression of TCF3 in Wilms' tumor and its regulatory role in kidney tumor cell viability, migration and apoptosis in vitro.
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Cancer Med Palladin promotes cancer stem cell-like properties in lung cancer by activating Wnt/Β-Catenin signaling |