TCF7L1 Polyclonal antibody

TCF7L1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14519-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

Transcription factor 7-like 1, TCF7L2, TCF4, TCF-3, TCF3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, mouse brain tissue, JAR cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14519-1-AP targets TCF7L1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, zebrafish
免疫原 TCF7L1 fusion protein Ag5983 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transcription factor 7-like 1 (T-cell specific, HMG-box)
别名 Transcription factor 7-like 1, TCF7L2, TCF4, TCF-3, TCF3
计算分子量 63 kDa
观测分子量 63-70 kDa
GenBank蛋白编号BC058894
基因名称 TCF7L1
Gene ID (NCBI) 83439
RRIDAB_2287033
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HCS4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TCF7L1 is one of TCF/LEF transcription factors that are mediators of the Wnt signaling pathway and are antagonized by the TGF-beta signaling pathway. It binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. It's necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis

实验方案

Product Specific Protocols
WB protocol for TCF7L1 antibody 14519-1-APDownload protocol
IHC protocol for TCF7L1 antibody 14519-1-APDownload protocol
IF protocol for TCF7L1 antibody 14519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Mol Immunol

TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal

Authors - Jing Wang
zebrafishIF

Nat Commun

Wnt/β-catenin signaling regulates VE-cadherin-mediated anastomosis of brain capillaries by counteracting S1pr1 signaling.

Authors - Kathleen Hübner
humanIF

Stem Cell Reports

Self-Renewing Trophoblast Organoids Recapitulate the Developmental Program of the Early Human Placenta.

Authors - Sandra Haider
humanIHC

Oncogenesis

TCF7L1 regulates cytokine response and neuroendocrine differentiation of prostate cancer.

Authors - Yu-Ching Wen
mouseWB

iScience

Suppression of preadipocyte determination by SOX4 limits white adipocyte hyperplasia in obesity

Authors - Ting He
humanWB,IF

Endocrinology

Wnt-dependent T-cell factor-4 controls human extravillous trophoblast motility.

Authors - Gudrun Meinhardt
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