验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, human liver tissue, human brain tissue, human heart tissue, HepG2 cells, mouse kidney tissue, mouse heart tissue, mouse placenta tissue, rat liver tissue, HEK-293 cells |
| Positive IP detected in | mouse liver tissue |
Planning an IHC experiment? We recommend our IHCeasy TCF4/TCF7L2 Ready-To-Use IHC Kit. TCF4/TCF7L2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13838-1-AP targets TCF4/TCF7L2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | TCF4/TCF7L2 fusion protein Ag4786 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transcription factor 7-like 2 (T-cell specific, HMG-box) |
| 别名 | HMG box transcription factor 4, hTCF 4, T cell factor 4, TCF 4, TCF4, TCF4/TCF7L2, TCF7L2, Transcription factor 7 like 2 |
| 计算分子量 | 619 aa, 68 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC032656 |
| 基因名称 | TCF4 |
| Gene ID (NCBI) | 6934 |
| RRID | AB_2199812 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NQB0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TCF4/TCF7L2 antibody 13838-1-AP | Download protocol |
| IP protocol for TCF4/TCF7L2 antibody 13838-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Immunol TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal | ||
Oncogene The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer. | ||
Sci Rep TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis. | ||
Front Cell Dev Biol Twist1 Contributes to the Maintenance of Some Biological Properties of Dermal Papilla Cells in vitro by Forming a Complex With Tcf4 and β-Catenin. | ||
Mol Cancer Res Wnt/β-Catenin Signaling Axis Is Required for TFEB-Mediated Gastric Cancer Metastasis and Epithelial-Mesenchymal Transition. | ||
J Biol Chem HBP1-mediated regulation of p21 through Mdm2/p53 and TCF4/EZH2 pathways and its impact on cell senescence and tumorigenesis. |
