验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells |
Positive IHC detected in | human liver tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
25931-1-AP targets TCHP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | TCHP fusion protein Ag23199 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | trichoplein, keratin filament binding |
别名 | Trichoplein keratin filament-binding protein, Protein TCHP, Mitostatin, Mitochondrial protein with oncostatic activity |
计算分子量 | 61 kDa |
观测分子量 | 61 kDa |
GenBank蛋白编号 | BC004285 |
基因名称 | TCHP |
Gene ID (NCBI) | 84260 |
RRID | AB_2880300 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9BT92 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for TCHP antibody 25931-1-AP | Download protocol |
IHC protocol for TCHP antibody 25931-1-AP | Download protocol |
IF protocol for TCHP antibody 25931-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Cell Mol Life Sci Requirement of NPHP5 in the hierarchical assembly of basal feet associated with basal bodies of primary cilia.
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Int J Mol Sci Primary Cilia Restrain PI3K-AKT Signaling to Orchestrate Human Decidualization
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J Cell Physiol Cell cycle arrest induced by trichoplein depletion is independent of cilia assembly.
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Nat Commun Uncovering structural themes across cilia microtubule inner proteins with implications for human cilia function |