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TCHP Polyclonal antibody

TCHP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25931-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA and More (1)

Trichoplein keratin filament-binding protein, Protein TCHP, Mitostatin, Mitochondrial protein with oncostatic activity

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman liver tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25931-1-AP targets TCHP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原 TCHP fusion protein Ag23199 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 trichoplein, keratin filament binding
别名 Trichoplein keratin filament-binding protein, Protein TCHP, Mitostatin, Mitochondrial protein with oncostatic activity
计算分子量 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC004285
基因名称 TCHP
Gene ID (NCBI) 84260
RRIDAB_2880300
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BT92
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TCHP antibody 25931-1-APDownload protocol
IHC protocol for TCHP antibody 25931-1-APDownload protocol
IF protocol for TCHP antibody 25931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Mol Life Sci

Requirement of NPHP5 in the hierarchical assembly of basal feet associated with basal bodies of primary cilia.

Authors - Delowar Hossain
  • KD Validated
humanWB

Int J Mol Sci

Primary Cilia Restrain PI3K-AKT Signaling to Orchestrate Human Decidualization

Authors - Bo Li
  • KD Validated
humanWB

J Cell Physiol

Cell cycle arrest induced by trichoplein depletion is independent of cilia assembly.

Authors - Min Huang
  • KD Validated
humanWB,IF

Nat Commun

Uncovering structural themes across cilia microtubule inner proteins with implications for human cilia function

Authors - Jens S Andersen
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