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  • Featured Product
  • KD/KO Validated

TCHP Polyclonal antibody

TCHP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25931-1-AP

Print datasheet

Synonyms

Trichoplein keratin filament-binding protein, Protein TCHP, Mitostatin, Mitochondrial protein with oncostatic activity

验证数据展示

  • WB analysis of MCF-7 using 25931-1-AP

    WB analysis of MCF-7 using 25931-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25931-1-AP (TCHP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 25931-1-AP

    IHC staining of human liver using 25931-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25931-1-AP (TCHP Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human small intestine using 25931-1-AP

    IHC staining of human small intestine using 25931-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25931-1-AP (TCHP Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of MCF-7 using 25931-1-AP

    IF Staining of MCF-7 using 25931-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TCHP antibody (25931-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman liver tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25931-1-AP targets TCHP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen TCHP fusion protein Ag23199 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name trichoplein, keratin filament binding
Synonyms Trichoplein keratin filament-binding protein, Protein TCHP, Mitostatin, Mitochondrial protein with oncostatic activity
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC004285
Gene Symbol TCHP
Gene ID (NCBI) 84260
RRIDAB_2880300
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BT92
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TCHP antibody 25931-1-APDownload protocol
IHC protocol for TCHP antibody 25931-1-APDownload protocol
IF protocol for TCHP antibody 25931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Mol Life Sci

Requirement of NPHP5 in the hierarchical assembly of basal feet associated with basal bodies of primary cilia.

Authors - Delowar Hossain
  • KD Validated
View Article
humanWB

Int J Mol Sci

Primary Cilia Restrain PI3K-AKT Signaling to Orchestrate Human Decidualization

Authors - Bo Li
  • KD Validated
View Article
humanWB

J Cell Physiol

Cell cycle arrest induced by trichoplein depletion is independent of cilia assembly.

Authors - Min Huang
  • KD Validated
View Article
humanWB,IF

Nat Commun

Uncovering structural themes across cilia microtubule inner proteins with implications for human cilia function

Authors - Jens S Andersen
View Article