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验证数据展示

  • WB analysis of Raji using 10475-1-AP

    WB analysis of Raji using 10475-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10475-1-AP (TCL1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 10475-1-AP

    IHC staining of human tonsillitis using 10475-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10475-1-AP (TCL1A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TCL1A antibody included
  • IHC staining of human tonsillitis using 10475-1-AP

    IHC staining of human tonsillitis using 10475-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10475-1-AP (TCL1A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TCL1A antibody included

TCL1A Polyclonal antibody

TCL1A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10475-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

TCL1, T-cell leukemia/lymphoma protein 1A, Oncogene TCL-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TCL1A Ready-To-Use IHC Kit. TCL1A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10475-1-AP targets TCL1A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen TCL1A fusion protein Ag0786 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name T-cell leukemia/lymphoma 1A
Synonyms TCL1, T-cell leukemia/lymphoma protein 1A, Oncogene TCL-1
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 14-16 kDa
GenBank Accession NumberBC005831
Gene Symbol TCL1A
Gene ID (NCBI) 8115
RRIDAB_2255885
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP56279
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TCL1A, also named as TCL1 and p14 TCL1, enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival. TCL1A immunodetection is an independent marker of adverse outcome that could be used in routine settings for the management of DLCL patients. TCL1A expression is correlated with shorter time to treatment in chronic lymphocytic leukemia cases and shorter lymphoma-specific survival in mantle cell lymphoma series. It is a potential therapeutic target.

实验方案

Product Specific Protocols
WB protocol for TCL1A antibody 10475-1-APDownload protocol
IHC protocol for TCL1A antibody 10475-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Biol Int

Protection of seven dibenzocyclooctadiene lignans from Schisandra chinensis against serum and glucose deprivation injury in SH-SY5Y cells.

Authors - Qun E
View Article