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验证数据展示

  • WB analysis of Ramos using 85129-5-RR

    WB analysis of Ramos using 85129-5-RR

    Ramos cells were subjected to SDS PAGE followed by western blot with 85129-5-RR (TCL1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of Ramos using 85129-5-RR

    IF Staining of Ramos using 85129-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Ramos cells using TCL1A antibody (85129-5-RR, Clone: 242623A9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85129-5-RR

    Affinity and Kinetic Characterization of 85129-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85129-5-RR against Human TCL1A were performed. The affinity constant is 0.185 nM.

TCL1A Recombinant antibody

TCL1A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85129-5-RR

产品说明书

CloneNo. 242623A9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

TCL1, T-cell leukemia/lymphoma protein 1A, Oncogene TCL-1, 242623A9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRamos cells
Positive IF/ICC detected inRamos cells
Planning an IHC experiment? We recommend our IHCeasy TCL1A Ready-To-Use IHC Kit. TCL1A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85129-5-RR targets TCL1A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 TCL1A fusion protein Ag0786 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 T-cell leukemia/lymphoma 1A
别名 TCL1, T-cell leukemia/lymphoma protein 1A, Oncogene TCL-1, 242623A9
计算分子量 13 kDa
观测分子量 15 kDa
GenBank蛋白编号BC005831
基因名称 TCL1A
Gene ID (NCBI) 8115
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP56279
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TCL1A, also named as TCL1 and p14 TCL1, enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival. TCL1A immunodetection is an independent marker of adverse outcome that could be used in routine settings for the management of DLCL patients. TCL1A expression is correlated with shorter time to treatment in chronic lymphocytic leukemia cases and shorter lymphoma-specific survival in mantle cell lymphoma series. It is a potential therapeutic target.

实验方案

Product Specific Protocols
WB protocol for TCL1A antibody 85129-5-RRDownload protocol
IF protocol for TCL1A antibody 85129-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols