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TCN2 Polyclonal antibody

TCN2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12157-1-AP

Print datasheet

Synonyms

D22S676, D22S750, TC 2, TC II, TC2, TCII, TCN2, Transcobalamin 2, Transcobalamin II

验证数据展示

  • WB analysis using 12157-1-AP

    WB analysis using 12157-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 12157-1-AP

    IHC staining of mouse small intestine using 12157-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TCN2 antibody included
  • IHC staining of mouse small intestine using 12157-1-AP

    IHC staining of mouse small intestine using 12157-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TCN2 antibody included
  • IHC staining of human pancreas cancer using 12157-1-AP

    IHC staining of human pancreas cancer using 12157-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TCN2 antibody included

经过测试的应用

Positive WB detected inBxPC-3 cells, mouse small intestine tissue, rat liver tissue, MCF-7 cells, U-87 MG cells
Positive IHC detected inmouse small intestine tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TCN2 Ready-To-Use IHC Kit. TCN2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12157-1-AP targets TCN2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine, cat
Immunogen TCN2 fusion protein Ag2825 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transcobalamin II; macrocytic anemia
Synonyms D22S676, D22S750, TC 2, TC II, TC2, TCII, TCN2, Transcobalamin 2, Transcobalamin II
Calculated Molecular Weight 400 aa, 44 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC011239
Gene Symbol TCN2
Gene ID (NCBI) 6948
RRIDAB_2303252
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20062
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)

实验方案

Product Specific Protocols
WB protocol for TCN2 antibody 12157-1-APDownload protocol
IHC protocol for TCN2 antibody 12157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
canine, catIHC

Oncotarget

Immunohistochemical quantification of the cobalamin transport protein, cell surface receptor and Ki-67 in naturally occurring canine and feline malignant tumors and in adjacent normal tissues.

Authors - Annette M Sysel
View Article
mouseIF

Int J Biol Macromol

CD320 expression and apical membrane targeting in renal and intestinal epithelial cells.

Authors - Yue Chen
View Article
humanIHC

Anticancer Res

Immunohistochemical Quantification of the Vitamin B12 Transport Protein (TCII), Cell Surface Receptor (TCII-R) and Ki-67 in Human Tumor Xenografts.

Authors - Sysel Annette M AM
View Article
ratWB

Mol Pharm

Investigation of Receptor-Mediated Cyanocobalamin (Vitamin B(12)) Transport across the Inner Blood-Retinal Barrier Using Fluorescence-Labeled Cyanocobalamin

Authors - Yuri Kinoshita
View Article