TCN2 Polyclonal antibody

TCN2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12157-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, ELISA and More (1)

D22S676, D22S750, TC 2, TC II, TC2, TCII, TCN2, Transcobalamin 2, Transcobalamin II

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inBxPC-3 cells, mouse small intestine tissue, rat liver tissue, MCF-7 cells, U-87 MG cells
Positive IHC detected inmouse small intestine tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TCN2 Ready-To-Use IHC Kit. TCN2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:300-1:600
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12157-1-AP targets TCN2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, canine, cat
免疫原 TCN2 fusion protein Ag2825 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transcobalamin II; macrocytic anemia
别名 D22S676, D22S750, TC 2, TC II, TC2, TCII, TCN2, Transcobalamin 2, Transcobalamin II
计算分子量 400 aa, 44 kDa
观测分子量 48 kDa
GenBank蛋白编号BC011239
基因名称 TCN2
Gene ID (NCBI) 6948
RRIDAB_2303252
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20062
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)

实验方案

Product Specific Protocols
WB protocol for TCN2 antibody 12157-1-APDownload protocol
IHC protocol for TCN2 antibody 12157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
canine, catIHC

Oncotarget

Immunohistochemical quantification of the cobalamin transport protein, cell surface receptor and Ki-67 in naturally occurring canine and feline malignant tumors and in adjacent normal tissues.

Authors - Annette M Sysel
mouseIF

Int J Biol Macromol

CD320 expression and apical membrane targeting in renal and intestinal epithelial cells.

Authors - Yue Chen
humanIHC

Anticancer Res

Immunohistochemical Quantification of the Vitamin B12 Transport Protein (TCII), Cell Surface Receptor (TCII-R) and Ki-67 in Human Tumor Xenografts.

Authors - Sysel Annette M AM
ratWB

Mol Pharm

Investigation of Receptor-Mediated Cyanocobalamin (Vitamin B(12)) Transport across the Inner Blood-Retinal Barrier Using Fluorescence-Labeled Cyanocobalamin

Authors - Yuri Kinoshita
Loading...