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TCN2 Monoclonal antibody

TCN2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G6D2

Cat no : 66377-1-Ig

Print datasheet

Synonyms

TC 2, TC II, TC2, TCII, TCN2, Transcobalamin 2, Transcobalamin II

验证数据展示

  • WB analysis of HUVEC using 66377-1-Ig

    WB analysis of HUVEC using 66377-1-Ig

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66377-1-Ig (TCN2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHUVEC cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy TCN2 Ready-To-Use IHC Kit. TCN2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66377-1-Ig targets TCN2 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen TCN2 fusion protein Ag2825 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name transcobalamin II; macrocytic anemia
Synonyms TC 2, TC II, TC2, TCII, TCN2, Transcobalamin 2, Transcobalamin II
Calculated Molecular Weight 400 aa, 44 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC011239
Gene Symbol TCN2
Gene ID (NCBI) 6948
RRIDAB_2881754
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20062
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)

实验方案

Product Specific Protocols
WB protocol for TCN2 antibody 66377-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols