验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells, A549 cells |
Planning an IHC experiment? We recommend our IHCeasy TCN2 Ready-To-Use IHC Kit. TCN2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66377-1-Ig targets TCN2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | TCN2 fusion protein Ag2825 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | transcobalamin II; macrocytic anemia |
| 别名 | TC 2, TC II, TC2, TCII, TCN2, Transcobalamin 2, Transcobalamin II |
| 计算分子量 | 400 aa, 44 kDa |
| 观测分子量 | 43 kDa |
| GenBank蛋白编号 | BC011239 |
| 基因名称 | TCN2 |
| Gene ID (NCBI) | 6948 |
| RRID | AB_2881754 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P20062 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TCN2 antibody 66377-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
