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TCOF1 Polyclonal antibody
TCOF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Neuro-2a cells, Jurkat cells |
| Positive IHC detected in | human colon cancer tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy TCOF1 Ready-To-Use IHC Kit. TCOF1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11003-1-AP targets TCOF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IP, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | TCOF1 fusion protein Ag1447 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Treacher Collins-Franceschetti syndrome 1 |
| Synonyms | treacle, MFD1 |
| Calculated Molecular Weight | 97 kDa, 144 kDa, 152 kDa |
| Observed Molecular Weight | 220 kDa |
| GenBank Accession Number | BC014559 |
| Gene Symbol | TCOF1 |
| Gene ID (NCBI) | 6949 |
| RRID | AB_2202516 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13428 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TCOF1 antibody 11003-1-AP | Download protocol |
| IHC protocol for TCOF1 antibody 11003-1-AP | Download protocol |
| IF protocol for TCOF1 antibody 11003-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Viral regulation of host cell biology by hijacking of the nucleolar DNA-damage response. | ||
Proc Natl Acad Sci U S A Treacher Collins syndrome TCOF1 protein cooperates with NBS1 in the DNA damage response.
| ||
Elife Multisite dependency of an E3 ligase controls monoubiquitylation-dependent cell fate decisions. | ||
Int J Mol Sci TRPV1 Activation Promotes β-arrestin2 Interaction with the Ribosomal Biogenesis Machinery in the Nucleolus:Implications for p53 Regulation and Neurite Outgrowth. | ||
Cancer Sci TCOF1 coordinates oncogenic activation and rRNA production and promotes tumorigenesis in HCC. |
