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- KD/KO Validated
TCP1 Polyclonal antibody
TCP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, mouse heart tissue, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human colon tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, MCF-7 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10320-1-AP targets TCP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IF, IP, CoIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | TCP1 fusion protein Ag0389 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | t-complex 1 |
| Synonyms | D6S230E, Chaperonin containing T-complex polypeptide 1 subunit 1, CCT-alpha, CCTa, CCT1 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | BC000665 |
| Gene Symbol | TCP1 |
| Gene ID (NCBI) | 6950 |
| RRID | AB_10694136 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P17987 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
0
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TCP1 antibody 10320-1-AP | Download protocol |
| IHC protocol for TCP1 antibody 10320-1-AP | Download protocol |
| IF protocol for TCP1 antibody 10320-1-AP | Download protocol |
| IP protocol for TCP1 antibody 10320-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol TRiC/CCT chaperonin is required for the folding and inhibitory effect of WDTC1 on adipogenesis | ||
J Alzheimers Dis Aging as a Precipitating Factor in Chronic Restraint Stress-Induced Tau Aggregation Pathology, and the Protective Effects of Rosmarinic Acid | ||
Virology Murine cytomegalovirus M72 promotes acute virus replication in vivo and is a substrate of the TRiC/CCT complex. | ||
J Agric Food Chem A Proteomic Study Reveals a Co-occurrence of Gallic Acid-induced Apoptosis and Glycolysis in B16F10 Melanoma Cells. |
