• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TCP1 Monoclonal antibody, PBS Only

TCP1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C2F6

Cat no : 68183-1-PBS

Print datasheet

Synonyms

D6S230E, Chaperonin containing T-complex polypeptide 1 subunit 1, CCT-alpha, CCTa, CCT1

验证数据展示

  • WB analysis using 68183-1-Ig (same clone as 68183-1-PBS)

    WB analysis using 68183-1-Ig (same clone as 68183-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68183-1-Ig (TCP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68183-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68183-1-Ig (same clone as 68183-1-PBS)

    IF Staining of HeLa using 68183-1-Ig (same clone as 68183-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TCP1 antibody (68183-1-Ig, Clone: 2C2F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68183-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68183-1-Ig (same clone as 68183-1-PBS)

    FC experiment of HeLa using 68183-1-Ig (same clone as 68183-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TCP1 (68183-1-Ig, Clone:2C2F6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68183-1-PBS in a different storage buffer formulation.

产品信息

68183-1-PBS targets TCP1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen TCP1 fusion protein Ag0389 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name t-complex 1
Synonyms D6S230E, Chaperonin containing T-complex polypeptide 1 subunit 1, CCT-alpha, CCTa, CCT1
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC000665
Gene Symbol TCP1
Gene ID (NCBI) 6950
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP17987
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C