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验证数据展示

  • WB analysis of mouse testis using 13709-1-AP

    WB analysis of mouse testis using 13709-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13709-1-AP (TCP10L antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 13709-1-AP

    IHC staining of human testis using 13709-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13709-1-AP (TCP10L antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 13709-1-AP

    IHC staining of human testis using 13709-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13709-1-AP (TCP10L antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 13709-1-AP

    IF Staining of HeLa using 13709-1-AP

    Immunofluorescent analysis of Hela cells, using 13709-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

TCP10L Polyclonal antibody

TCP10L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13709-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

TCP10-like, TCP10A-1, T-complex protein 10A-1, T-complex protein 10A homolog 1, PRED77

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13709-1-AP targets TCP10L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 TCP10L fusion protein Ag4646 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 t-complex 10 (mouse)-like
别名 TCP10-like, TCP10A-1, T-complex protein 10A-1, T-complex protein 10A homolog 1, PRED77
计算分子量 215 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC033855
基因名称 TCP10L
Gene ID (NCBI) 140290
RRIDAB_2255905
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TDR4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TCP10L antibody 13709-1-APDownload protocol
IHC protocol for TCP10L antibody 13709-1-APDownload protocol
IF protocol for TCP10L antibody 13709-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols