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验证数据展示

  • WB analysis using 83227-1-RR

    WB analysis using 83227-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83227-1-RR (TCP11L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 83227-1-RR

    FC experiment of A431 using 83227-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug TCP11L2 Recombinant antibody (83227-1-RR, Clone:240096F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of U-251 using 83227-1-RR

    IF Staining of U-251 using 83227-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using TCP11L2 antibody (83227-1-RR, Clone: 240096F6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Affinity and Kinetic Characterization of 83227-1-RR

    Affinity and Kinetic Characterization of 83227-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83327-1-RR against Human TCP11L2 were performed. The affinity constant is below 1 pM.

TCP11L2 Recombinant antibody

TCP11L2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83227-1-RR

产品说明书

CloneNo. 240096F6

宿主/亚型

Rabbit / IgG

种属反应性

human, canine

应用

WB, IF/ICC, FC (Intra), ELISA

别名

T-complex protein 11-like protein 2, t complex 11 (mouse) like 2, 240096F6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, MDCK cells, U251 cells
Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inA431 cells, SH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83227-1-RR targets TCP11L2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, canine samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, canine
免疫原 TCP11L2 fusion protein Ag11185 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 t-complex 11 (mouse)-like 2
别名 T-complex protein 11-like protein 2, t complex 11 (mouse) like 2, 240096F6
计算分子量 519 aa, 58 kDa
观测分子量60 kDa
GenBank蛋白编号BC033617
基因名称 TCP11L2
Gene ID (NCBI) 255394
RRIDAB_3670910
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N4U5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TCP11L2 is a protein containing a serine-rich region in its N-terminal region. It is a member of the TCP11 family, which includes SOK1, TCP11L1, and TCP11L2. TCP11L2 has a role in the migration and differentiation of skeletal muscle-derived satellite cells. It has been shown that Tcp11l2 expression gradually increased during the differentiation of bovine muscle-derived satellite cells. (PMID: 32017087)

实验方案

Product Specific Protocols
WB protocol for TCP11L2 antibody 83227-1-RRDownload protocol
IF protocol for TCP11L2 antibody 83227-1-RRDownload protocol
FC protocol for TCP11L2 antibody 83227-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols