验证数据展示
经过测试的应用
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:525-1:2100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82120-1-RR targets TCTN2 in IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | IHC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | TCTN2 fusion protein Ag10725 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | tectonic family member 2 |
Synonyms | Tectonic-2, tectonic family member 2, Tectonic 2, TECT2, C12orf38 |
Calculated Molecular Weight | 697 aa, 77 kDa |
GenBank Accession Number | BC009112 |
Gene Symbol | TCTN2 |
Gene ID (NCBI) | 79867 |
RRID | AB_3670517 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q96GX1 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TCTN2 (tectonic-2 or TECT2) is a type I membrane protein that belongs to the tectonic family, which consists of TCTN1, TCTN2 and TCTN3. Studies in mice suggest that tectonic may be involved in Hedgehog (Hh) signaling, and essential for ciliogenesis. Tectonic is component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. It exists two spilcing isoforms. The first aberrant transcript introduces 104 base pair from intron 13 and would delete 196 original amino acid, introduce two novel amino acids, and prematurely truncate the 697aa protein at residue number 504. The second transcript lacks exon 14 and would delete 195 original amino acids, introduce four novel amino acids, and prematurely truncate the 697 aa protein at residue number 507. The molecular weight of two isoforms are about 50kDa. We detected the 50kDa and 90kDa protein by immunoprecipitaion, but 50kDa protein may be the splicing isoform or non-specific band.
实验方案
Product Specific Protocols | |
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IHC protocol for TCTN2 antibody 82120-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |