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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66707-1-Ig (same clone as 66707-1-PBS)

    WB analysis using 66707-1-Ig (same clone as 66707-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66707-1-Ig (TDG antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66707-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66707-1-Ig (same clone as 66707-1-PBS)

    IHC staining of human breast cancer using 66707-1-Ig (same clone as 66707-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66707-1-Ig (TDG antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66707-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TDG antibody included
  • IF Staining of HepG2 using 66707-1-Ig (same clone as 66707-1-PBS)

    IF Staining of HepG2 using 66707-1-Ig (same clone as 66707-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66707-1-Ig (TDG antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66707-1-PBS in a different storage buffer formulation.

TDG Monoclonal antibody, PBS Only

TDG Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66707-1-PBS

产品说明书

CloneNo. 1G2E2

宿主/亚型

Mouse / IgG2b

种属反应性

Human, rat, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

TDG, thymine DNA glycosylase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TDG Ready-To-Use IHC Kit. TDG primary antibody included.

产品信息

66707-1-PBS targets TDG in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, rat, mouse
免疫原 TDG fusion protein Ag27408 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 thymine-DNA glycosylase
别名 TDG, thymine DNA glycosylase
计算分子量 410 aa, 46 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC037557
基因名称 TDG
Gene ID (NCBI) 6996
RRIDAB_2882059
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13569
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TDG belongs to the TDG/mug DNA glycosylase family. TDG corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. RNF4 interacts with and requires the base excision repair enzymes TDG and APE1 for active demethylation (PMID:20696907). TDG is modified by SUMO-1 and SUMO-2/3.The molecular weight of non-modified TDG is 46 kDa and modified TDG is 55-60 kDa.