验证数据展示
经过测试的应用
| Positive WB detected in | MCF7 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10641-1-AP targets TDP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | TDP1 fusion protein Ag0964 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tyrosyl-DNA phosphodiesterase 1 |
| 别名 | Tyrosyl-DNA phosphodiesterase 1, Tyr-DNA phosphodiesterase 1, Tyr DNA phosphodiesterase 1, EC:3.1.4.- |
| 计算分子量 | 34 kDa, 68 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC006083 |
| 基因名称 | TDP1 |
| Gene ID (NCBI) | 55775 |
| RRID | AB_2240520 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NUW8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tyrosyl DNA phosphodiesterase 1 (TDP1) is an enzyme capable of hydrolyzing phosphodiester bonds between tyrosine and the 3-phosphate of DNA, which are typically generated in a transient manner by DNA topoisomerase I (topo I) and it appears to be more mobile and diffusible than topo I, and it has a different distribution in the nucleus (PMID:15494395). TDP1 is involed in protecting cells against oxidative DNA damage, and with the impaired ability of TDP1-deficient cells to remove 3-phosphoglycolate (PMID:21041670). Defects in TDP1 are the cause of spinocerebellar ataxia autosomal recessive with axonal neuropathy (SCAN1)(PMID:17948061).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TDP1 antibody 10641-1-AP | Download protocol |
| IHC protocol for TDP1 antibody 10641-1-AP | Download protocol |
| IP protocol for TDP1 antibody 10641-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Pharmacol Role of tyrosyl-DNA phosphodiesterase 1 and inter-players in regulation of tumor cell sensitivity to topoisomerase I inhibition. | ||
DNA Repair (Amst) TDP1 is required for efficient non-homologous end joining in human cells.
| ||
Int J Mol Sci Combined PARP and Dual Topoisomerase Inhibition Potentiates Genome Instability and Cell Death in Ovarian Cancer | ||
Anal Chem Spike-In Proteome Enhances Data-Independent Acquisition for Thermal Proteome Profiling |
