• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

TDRD3 Polyclonal antibody

TDRD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13359-1-AP

Print datasheet

Synonyms

Tudor domain-containing protein 3, tudor domain containing 3

验证数据展示

  • IHC staining of mouse brain using 13359-1-AP

    IHC staining of mouse brain using 13359-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13359-1-AP (TDRD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TDRD3 antibody included
  • IHC staining of mouse brain using 13359-1-AP

    IHC staining of mouse brain using 13359-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13359-1-AP (TDRD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TDRD3 antibody included
  • WB analysis of HEK-293 using 13359-1-AP

    WB analysis of HEK-293 using 13359-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13359-1-AP (TDRD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 13359-1-AP

    WB analysis of HEK-293 using 13359-1-AP

    WB result of TDRD3 antibody (13359-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDRD3 transfected HEK-293 cells.

  • IP experiment of HeLa using 13359-1-AP

    IP experiment of HeLa using 13359-1-AP

    IP result of anti-TDRD3 (IP:13359-1-AP, 3ug; Detection:13359-1-AP 1:1000) with HeLa cells lysate 3800ug.

  • IF Staining of HeLa using 13359-1-AP

    IF Staining of HeLa using 13359-1-AP

    Immunofluorescent analysis of HeLa cells using 13359-1-AP (TDRD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TDRD3 Ready-To-Use IHC Kit. TDRD3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13359-1-AP targets TDRD3 in WB, IHC, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, chIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TDRD3 fusion protein Ag4174 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tudor domain containing 3
Synonyms Tudor domain-containing protein 3, tudor domain containing 3
Calculated Molecular Weight 651 aa, 73 kDa
Observed Molecular Weight 73 kDa, 83 kDa
GenBank Accession NumberBC030514
Gene Symbol TDRD3
Gene ID (NCBI) 81550
RRIDAB_2202810
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H7E2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TDRD3 (Tudor domain-containing protein 3) contains tudor domain which is an approximately 60-amino acid structure motif. TDRD3 functions as a scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. TDRD3 is a transcriptional coactivator that promotes transcription by binding methylarginine marks on histone tails. TDRD3 also possesses an oligosaccharide/nucleotide binding fold and an ubiquitin associated domain capable of binding tetra-ubiquitin, and it is reported to associate with polyribosomes in HeLa cells.

实验方案

Product Specific Protocols
WB protocol for TDRD3 antibody 13359-1-APDownload protocol
IHC protocol for TDRD3 antibody 13359-1-APDownload protocol
IF protocol for TDRD3 antibody 13359-1-APDownload protocol
IP protocol for TDRD3 antibody 13359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanchIP

Theranostics

A hypermethylation strategy utilized by enhancer-bound CARM1 to promote estrogen receptor α-dependent transcriptional activation and breast carcinogenesis.

Authors - Bing-Ling Peng
View Article
humanWB

Int J Biol Macromol

PRMT1 and TDRD3 promote stress granule assembly by rebuilding the protein-RNA interaction network

Authors - Mengtong Qin
View Article