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TEAD3 Monoclonal antibody

TEAD3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E6A12

Cat no : 68464-1-Ig

Print datasheet

Synonyms

DTEF 1, ETFR 1, TEA domain family member 3, TEAD 3, TEAD3, TEAD5, TEF 5, TEF5

验证数据展示

  • WB analysis using 68464-1-Ig

    WB analysis using 68464-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68464-1-Ig (TEAD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68464-1-Ig

    WB analysis using 68464-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68464-1-Ig (TEAD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inHCT 116 cells, JAR cells, HepG2 cells, U2OS cells, pig heart tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68464-1-Ig targets TEAD3 in WB, ELISA applications and shows reactivity with Human, pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, pig
Immunogen TEAD3 fusion protein Ag4407 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TEA domain family member 3
Synonyms DTEF 1, ETFR 1, TEA domain family member 3, TEAD 3, TEAD3, TEAD5, TEF 5, TEF5
Calculated Molecular Weight 435 aa, 49 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC027877
Gene Symbol TEAD3
Gene ID (NCBI) 7005
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99594
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TEAD3, others named transcription enhancer factor 5(TEF5), is a member of the family of transcription factors that plays a key role in the Hippo signaling pathway, suggesting involvement in organ and tumor suppression by restricting proliferation and promoting. TEAD3 contains the TEA/ATTS DNA-binding domain. Human TEAD3 has a strong mRNA expression in the placenta and choriocarcinoma cells(JEG-3) but is low in Hela, HepG2, and MCF-7.

实验方案

Product Specific Protocols
WB protocol for TEAD3 antibody 68464-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols