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验证数据展示

  • WB analysis of MDA-MB-231 using 84932-4-RR

    WB analysis of MDA-MB-231 using 84932-4-RR

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 84932-4-RR (TEAD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84932-4-RR

    IF Staining of HeLa using 84932-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TEAD4 antibody (84932-4-RR, Clone: 242496B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • WB analysis of BGC-823 using 84932-4-RR

    WB analysis of BGC-823 using 84932-4-RR

    BGC-823 cells were subjected to SDS PAGE followed by western blot with 84932-4-RR (TEAD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84932-4-RR

    Affinity and Kinetic Characterization of 84932-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84932-4-RR against Human TEAD4 were performed. The affinity constant is 0.205 nM.

TEAD4 Recombinant antibody

TEAD4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84932-4-RR

产品说明书

CloneNo. 242496B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

TEA domain family member 4, TCF13L1, RTEF1, hRTEF 1B, EFTR 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMDA-MB-231 cells, BGC-823 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84932-4-RR targets TEAD4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 TEAD4 fusion protein Ag3059 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TEA domain family member 4
别名 TEA domain family member 4, TCF13L1, RTEF1, hRTEF 1B, EFTR 2
计算分子量 434 aa, 48 kDa
观测分子量50-55 kDa
GenBank蛋白编号BC015497
基因名称 TEAD4
Gene ID (NCBI) 7004
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15561
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TEAD4 belongs to a protein family that shares the TEA/ATTS domain. The transcriptional activity of TEAD4 required several regions of the proteins, a TBP-associated factor, and a limiting transcriptional intermediary factor. TEAD4 also performs an essential role in the Hippo signaling pathway. TEAD4 regulates gene expression of YAP1 and WWTR1/TAZ, thus mediating cell proliferation, migration, and epithelial-mesenchymal transition induction. The calculated molecular weight of TEAD4 is 48 kDa, but the molecular weight of TEAD4 is detected at about 50-55 kDa.

实验方案

Product Specific Protocols
WB protocol for TEAD4 antibody 84932-4-RRDownload protocol
IF protocol for TEAD4 antibody 84932-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols