验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, mouse brain tissue, rat testis tissue |
| Positive IHC detected in | human trachea tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13518-1-AP targets TEKT2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, bovine |
| 免疫原 | TEKT2 fusion protein Ag4457 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tektin 2 (testicular) |
| 别名 | Tektin-B1, Tektin-2, Tektin B1, Tektin 2, TEKTB1 |
| 计算分子量 | 430 aa, 50 kDa |
| 观测分子量 | 54 kDa |
| GenBank蛋白编号 | BC035620 |
| 基因名称 | TEKT2 |
| Gene ID (NCBI) | 27285 |
| RRID | AB_2203328 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UIF3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TEKT2 antibody 13518-1-AP | Download protocol |
| IHC protocol for TEKT2 antibody 13518-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Curr Biol CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement | ||
Cell Mol Life Sci Tektin bundle interacting protein, TEKTIP1, functions to stabilize the tektin bundle and axoneme in mouse sperm flagella | ||
Front Cell Dev Biol TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice. | ||
Asian-Australas J Anim Sci Expression pattern of prohibitin, capping actin protein of muscle Z-line beta subunit and tektin-2 gene in Murrah buffalo sperm and its relationship with sperm motility. | ||
Cell Mol Life Sci CCDC146 is required for sperm flagellum biogenesis and male fertility in mice | ||
Dev Dyn Proteomic profiling of TBC1 domain family member 21-null sperms reveals the critical roles of TEKT 1 in their tail defects |
