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TEKT4 Polyclonal antibody

TEKT4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 17058-1-AP

Print datasheet

Synonyms

TEKT4, tektin 4

验证数据展示

  • WB analysis of MDA-MB-453s using 17058-1-AP

    WB analysis of MDA-MB-453s using 17058-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 17058-1-AP (TEKT4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 17058-1-AP

    IHC staining of human kidney using 17058-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17058-1-AP (TEKT4 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HeLa using 17058-1-AP

    IP experiment of HeLa using 17058-1-AP

    IP result of anti-TEKT4 (IP:17058-1-AP, 4ug; Detection:17058-1-AP 1:500) with HeLa cells lysate 1200ug.

经过测试的应用

Positive WB detected inMDA-MB-453s cells, human testis tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human lung tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17058-1-AP targets TEKT4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen TEKT4 fusion protein Ag10772 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tektin 4
Synonyms TEKT4, tektin 4
Calculated Molecular Weight 435 aa, 51 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC021716
Gene Symbol TEKT4
Gene ID (NCBI) 150483
RRIDAB_2203334
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WW24
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TEKT4 antibody 17058-1-APDownload protocol
IHC protocol for TEKT4 antibody 17058-1-APDownload protocol
IP protocol for TEKT4 antibody 17058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF,CoIP

Nat Commun

Enriched variations in TEKT4 and breast cancer resistance to paclitaxel.

Authors - Yi-Zhou Jiang
View Article
mouseWB,IP

Cell Mol Life Sci

Tektin bundle interacting protein, TEKTIP1, functions to stabilize the tektin bundle and axoneme in mouse sperm flagella

Authors - Xin-Yan Geng
View Article
mouseIF

Dev Dyn

Proteomic profiling of TBC1 domain family member 21-null sperms reveals the critical roles of TEKT 1 in their tail defects

Authors - Pei-Yi Pan
View Article