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TEKT5 Polyclonal antibody

TEKT5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26108-1-AP

Print datasheet

Synonyms

TEKT5, tektin 5

验证数据展示

  • WB analysis using 26108-1-AP

    WB analysis using 26108-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26108-1-AP (TEKT5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat testis using 26108-1-AP

    IHC staining of rat testis using 26108-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 26108-1-AP (TEKT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of COLO 320 using 26108-1-AP

    WB analysis of COLO 320 using 26108-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 26108-1-AP (TEKT5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inCOLO 320 cells, HepG2 cells, mouse brain tissue, NIH/3T3 cells, mouse testis tissue
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26108-1-AP targets TEKT5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TEKT5 fusion protein Ag23295 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tektin 5
Synonyms TEKT5, tektin 5
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC130336
Gene Symbol TEKT5
Gene ID (NCBI) 146279
RRIDAB_2880381
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96M29
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TEKT5 antibody 26108-1-APDownload protocol
IHC protocol for TEKT5 antibody 26108-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols