验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, Y79 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15975-1-AP targets TELO2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | TELO2 fusion protein Ag8763 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TEL2, telomere maintenance 2, homolog (S. cerevisiae) |
| 别名 | TEL2, Protein clk-2 homolog, Protein clk 2 homolog, KIAA0683, hCLK2 |
| 计算分子量 | 837 aa, 92 kDa |
| 观测分子量 | 92 kDa |
| GenBank蛋白编号 | BC017188 |
| 基因名称 | TELO2 |
| Gene ID (NCBI) | 9894 |
| RRID | AB_2203337 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y4R8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TELO2 gene encodes a telomere length regulation protein TEL2 homolog. TELO2 may be involved in telomere length regulation and can form TTT complex with TTI1 and TTI2. TTT complex is required to stabilize protein levels of PIKK family proteins and is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), ultraviolet (UV) and MMC. The activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals, can be promoted, stabilized and maintained by TELO2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TELO2 antibody 15975-1-AP | Download protocol |
| IHC protocol for TELO2 antibody 15975-1-AP | Download protocol |
| IF protocol for TELO2 antibody 15975-1-AP | Download protocol |
| IP protocol for TELO2 antibody 15975-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol SCFFbxo9 and CK2 direct the cellular response to growth factor withdrawal via Tel2/Tti1 degradation and promote survival in multiple myeloma. | ||
Mol Cell The IMiD target CRBN determines HSP90 activity toward transmembrane proteins essential in multiple myeloma. | ||
Mol Cell Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex. | ||
Sci Adv Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells. | ||
Genes Dev A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability. | ||
Am J Hum Genet A Syndromic Intellectual Disability Disorder Caused by Variants in TELO2, a Gene Encoding a Component of the TTT Complex. |
