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验证数据展示

  • IHC staining of mouse brain using 22612-1-AP

    IHC staining of mouse brain using 22612-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22612-1-AP (TET3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 22612-1-AP

    IF Staining of U2OS using 22612-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TET3 antibody (22612-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

TET3 Polyclonal antibody

TET3 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 22612-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

tet oncogene family member 3, Methylcytosine dioxygenase TET3, KIAA0401, EC:1.14.11.80

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22612-1-AP targets TET3 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 TET3 fusion protein Ag18379 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tet oncogene family member 3
别名 tet oncogene family member 3, Methylcytosine dioxygenase TET3, KIAA0401, EC:1.14.11.80
计算分子量 1660 aa, 179 kDa
GenBank蛋白编号BC022243
基因名称 TET3
Gene ID (NCBI) 200424
RRIDAB_3085700
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDO43151
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ten-eleven translocation protein 3 (TET3) belongs to the family of ten-eleven translocations (TETs) which induce DNA demethylation and gene regulation at the epigenetic level by converting 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) (PMID: 31656201). In ovarian cancer, TET3 is reported as an oncogene or tumor suppressor during tumorigenesis and overexpressed in ovarian cancer tissues (PMID: 27141829).

实验方案

Product Specific Protocols
IHC protocol for TET3 antibody 22612-1-APDownload protocol
IF protocol for TET3 antibody 22612-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols