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TET3 Polyclonal antibody

TET3 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22612-1-AP

Print datasheet

Synonyms

tet oncogene family member 3, Methylcytosine dioxygenase TET3, KIAA0401, EC:1.14.11.80

验证数据展示

  • IHC staining of mouse brain using 22612-1-AP

    IHC staining of mouse brain using 22612-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22612-1-AP (TET3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 22612-1-AP

    IF Staining of U2OS using 22612-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TET3 antibody (22612-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22612-1-AP targets TET3 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen TET3 fusion protein Ag18379 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tet oncogene family member 3
Synonyms tet oncogene family member 3, Methylcytosine dioxygenase TET3, KIAA0401, EC:1.14.11.80
Calculated Molecular Weight 1660 aa, 179 kDa
GenBank Accession NumberBC022243
Gene Symbol TET3
Gene ID (NCBI) 200424
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDO43151
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ten-eleven translocation protein 3 (TET3) belongs to the family of ten-eleven translocations (TETs) which induce DNA demethylation and gene regulation at the epigenetic level by converting 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) (PMID: 31656201). In ovarian cancer, TET3 is reported as an oncogene or tumor suppressor during tumorigenesis and overexpressed in ovarian cancer tissues (PMID: 27141829).

实验方案

Product Specific Protocols
IHC protocol for TET3 antibody 22612-1-APDownload protocol
IF protocol for TET3 antibody 22612-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols