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Species Reactivity
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验证数据展示

  • WB analysis using 27150-1-AP

    WB analysis using 27150-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27150-1-AP (TET3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

TET3 Polyclonal antibody

TET3 Polyclonal Antibody for WB, ELISA
Cat No. 27150-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

tet oncogene family member 3, Methylcytosine dioxygenase TET3, KIAA0401, EC:1.14.11.80

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, SKOV-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27150-1-AP targets TET3 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen TET3 fusion protein Ag25877 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tet oncogene family member 3
Synonyms tet oncogene family member 3, Methylcytosine dioxygenase TET3, KIAA0401, EC:1.14.11.80
Calculated Molecular Weight 1660 aa, 179 kDa
Observed Molecular Weight180 kDa
GenBank Accession NumberBC022243
Gene Symbol TET3
Gene ID (NCBI) 200424
RRIDAB_3669585
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43151
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ten-eleven translocation protein 3 (TET3) belongs to the family of ten-eleven translocations (TETs) which induce DNA demethylation and gene regulation at the epigenetic level by converting 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) (PMID: 31656201). In ovarian cancer, TET3 is reported as an oncogene or tumor suppressor during tumorigenesis and overexpressed in ovarian cancer tissues (PMID: 27141829).

实验方案

Product Specific Protocols
WB protocol for TET3 antibody 27150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols