验证数据展示
经过测试的应用
| Positive WB detected in | rat testis tissue |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
24585-1-AP targets TEX15 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | TEX15 fusion protein Ag20170 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | testis expressed 15 |
| 别名 | Cancer/testis antigen 42, CT42, testis expressed 15, TEX15 |
| 计算分子量 | 2789 aa, 315 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | BC141841 |
| 基因名称 | TEX15 |
| Gene ID (NCBI) | 56154 |
| RRID | AB_2879623 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BXT5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TEX15 also named as Cancer/testis antigen 42 is a 2789 amino acid protein, which is expressed exclusively in testicular and cancerous tissues and may be involved in the development and metastasis of several different types of tumors. TEX15 exists as a 1279 amino acid protein in the mouse and rat testis. The molecular weight of mouse/rat TEX15 is 150 kDa (PMID: 26959739).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TEX15 antibody 24585-1-AP | Download protocol |
| IHC protocol for TEX15 antibody 24585-1-AP | Download protocol |
| IP protocol for TEX15 antibody 24585-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Toxicol Lett Downregulation of vdac2 inhibits spermatogenesis via JNK and P53 signalling in mice exposed to cadmium. | ||
Fertil Steril Differential expression of miR-23a/b-3p and its target genes in male patients with subfertility. |
