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验证数据展示

  • WB analysis using 82745-1-RR

    WB analysis using 82745-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82745-1-RR (TFAM antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82745-1-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 82745-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82745-1-RR (TFAM antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 82745-1-RR

    IF Staining of HepG2 using 82745-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (82745-1-RR, Clone: 4L4 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • Affinity and Kinetic Characterization of 82745-1-RR

    Affinity and Kinetic Characterization of 82745-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82745-1-RR against Human TFAM were performed. The affinity constant is 45.7 pM.

TFAM Recombinant antibody

TFAM Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 82745-1-RR

产品说明书

CloneNo. 4L4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Transcription factor 6-like 2, TCF-6, TCF6, mtTFA, MtTF1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82745-1-RR targets TFAM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 TFAM fusion protein Ag18299 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transcription factor A, mitochondrial
别名 Transcription factor 6-like 2, TCF-6, TCF6, mtTFA, MtTF1
计算分子量 246 aa, 29 kDa
观测分子量24-25 kDa
GenBank蛋白编号BC126366
基因名称 TFAM
Gene ID (NCBI) 7019
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ00059
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.

实验方案

Product Specific Protocols
WB protocol for TFAM antibody 82745-1-RRDownload protocol
IHC protocol for TFAM antibody 82745-1-RRDownload protocol
IF protocol for TFAM antibody 82745-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols