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TFAP2A/AP-2 Monoclonal antibody, PBS Only

TFAP2A/AP-2 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B12F11

Cat no : 67076-1-PBS

Print datasheet

Synonyms

TFAP2A, TFAP2A,AP-2, AP-2, AP2, AP 2alpha

验证数据展示

  • WB analysis of L02 using 67076-1-Ig (same clone as 67076-1-PBS)

    WB analysis of L02 using 67076-1-Ig (same clone as 67076-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67076-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67076-1-Ig (same clone as 67076-1-PBS)

    IHC staining of human breast cancer using 67076-1-Ig (same clone as 67076-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67076-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TFAP2A antibody included
  • FC experiment of A431 using 67076-1-Ig (same clone as 67076-1-PBS)

    FC experiment of A431 using 67076-1-Ig (same clone as 67076-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human TFAP2A/AP-2 (67076-1-Ig, Clone:1B12F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67076-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TFAP2A Ready-To-Use IHC Kit. TFAP2A primary antibody included.

产品信息

67076-1-PBS targets TFAP2A/AP-2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TFAP2A/AP-2 fusion protein Ag4112 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
Synonyms TFAP2A, TFAP2A,AP-2, AP-2, AP2, AP 2alpha
Calculated Molecular Weight 431 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC017754
Gene Symbol TFAP2A/AP-2
Gene ID (NCBI) 7020
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05549
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The activator protein-2 (AP-2) family of transcription factors comprises five 52-kDa isoforms (AP-2α, AP-2β, AP-2γ, AP-2δ, and AP-2ε), which share a common structure: a proline/glutamine-rich transactivation domain in the N-terminal region and a helix-span-helix domain in the C-terminal region, which mediates dimerization and site-specific DNA binding. Depending on the cellular context, the AP-2 transcription factors are individually associated either with cell differentiation and development or with cancer progression/regression. [PMID:21966377] TFAP2A (AP-2-alpha) is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation [PMID:11694877]