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TFAP2B Polyclonal antibody

TFAP2B Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 13183-1-AP

Print datasheet

Synonyms

AP 2B, AP2 B, AP2 beta, TFAP2B, Transcription factor AP 2 beta

验证数据展示

  • WB analysis of MCF-7 using 13183-1-AP

    WB analysis of MCF-7 using 13183-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13183-1-AP (TFAP2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 13183-1-AP

    IP experiment of MCF-7 using 13183-1-AP

    IP result of anti-TFAP2B (IP:13183-1-AP, 4ug; Detection:13183-1-AP 1:500) with MCF-7 cells lysate 800ug.

经过测试的应用

Positive WB detected inMCF7 cells, MCF-7 cells
Positive IP detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13183-1-AP targets TFAP2B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TFAP2B fusion protein Ag3853 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transcription factor AP-2 beta (activating enhancer binding protein 2 beta)
Synonyms AP 2B, AP2 B, AP2 beta, TFAP2B, Transcription factor AP 2 beta
Calculated Molecular Weight 449 aa, 49 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC037225
Gene Symbol TFAP2B
Gene ID (NCBI) 7021
RRIDAB_2286992
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92481
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TFAP2B antibody 13183-1-APDownload protocol
IP protocol for TFAP2B antibody 13183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncol Lett

miR-142-5p promotes renal cell tumorigenesis by targeting TFAP2B.

Authors - Maoshu Zhu
View Article