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TFAP2E Polyclonal antibody

TFAP2E Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25829-1-AP

Print datasheet

Synonyms

AP2 epsilon, AP2E, TFAP2E

验证数据展示

  • IHC staining of human skin cancer using 25829-1-AP

    IHC staining of human skin cancer using 25829-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 25829-1-AP (TFAP2E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of A375 using 25829-1-AP

    WB analysis of A375 using 25829-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 25829-1-AP (TFAP2E Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of A375 using 25829-1-AP

    IF Staining of A375 using 25829-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using 25829-1-AP (TFAP2E antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA375 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

The immunogen of 25829-1-AP is TFAP2E Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen TFAP2E fusion protein Ag23023 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transcription factor AP-2 epsilon (activating enhancer binding protein 2 epsilon)
Synonyms AP2 epsilon, AP2E, TFAP2E
Calculated Molecular Weight 442 aa, 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC041175
Gene Symbol TFAP2E
Gene ID (NCBI) 339488
RRIDAB_2880258
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6VUC0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TFAP2E antibody 25829-1-APDownload protocol
IHC protocol for TFAP2E antibody 25829-1-APDownload protocol
IF protocol for TFAP2E antibody 25829-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Elife

Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2ε in vomeronasal sensory neurons

Authors - Jennifer M Lin
View Article