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验证数据展示

  • IP experiment of HepG2 using 16604-1-AP

    IP experiment of HepG2 using 16604-1-AP

    IP result of anti-TFB1M (IP:16604-1-AP, 4ug; Detection:16604-1-AP 1:1000) with HepG2 cells lysate 1085 ug.

  • IF Staining of HepG2 using 16604-1-AP

    IF Staining of HepG2 using 16604-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFB1M antibody (16604-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

TFB1M Polyclonal antibody

TFB1M Polyclonal Antibody for IF/ICC, IP, ELISA
Cat No. 16604-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, IP, ELISA

别名

h mtTFB1, h mtTFB, EC:2.1.1.-, CGI-75, CGI75

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16604-1-AP targets TFB1M in IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 TFB1M fusion protein Ag9785 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transcription factor B1, mitochondrial
别名 h mtTFB1, h mtTFB, EC:2.1.1.-, CGI-75, CGI75
计算分子量 346 aa, 40 kDa
观测分子量39 kDa
GenBank蛋白编号BC017788
基因名称 TFB1M
Gene ID (NCBI) 51106
RRIDAB_3085501
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WVM0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for TFB1M antibody 16604-1-APDownload protocol
IP protocol for TFB1M antibody 16604-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols