• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TFE3 Monoclonal antibody

TFE3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E3D7

Cat no : 67319-1-Ig

Print datasheet

Synonyms

bHLHe33, RCCP2, TFE3, TFEA, Transcription factor E3

验证数据展示

  • WB analysis using 67319-1-Ig

    WB analysis using 67319-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67319-1-Ig (TFE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human malignant melanoma using 67319-1-Ig

    IHC staining of human malignant melanoma using 67319-1-Ig

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 67319-1-Ig (TFE3 antibody) at dilution of 1:200 (under 40x lens)..

    Ready-to-use IHC kits available with TFE3 antibody included

经过测试的应用

Positive WB detected inHEK-293 cells, C2C12 cells, pig spleen tissue, HeLa cells, Raji cells, K-562 cells, rat spleen tissue
Positive IHC detected inhuman malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TFE3 Ready-To-Use IHC Kit. TFE3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67319-1-Ig targets TFE3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen TFE3 fusion protein Ag6320 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name transcription factor binding to IGHM enhancer 3
Synonyms bHLHe33, RCCP2, TFE3, TFEA, Transcription factor E3
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC026027
Gene Symbol TFE3
Gene ID (NCBI) 7030
RRIDAB_2882579
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19532
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TFE3 antibody 67319-1-IgDownload protocol
IHC protocol for TFE3 antibody 67319-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols