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TFEB Monoclonal antibody, PBS Only (Capture)

TFEB Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C2D9

Cat no : 68632-1-PBS

Print datasheet

Synonyms

TCFEB, Class E basic helix-loop-helix protein 35, bHLHe35, AlphaTFEB, Alpha TFEB

验证数据展示

  • Cytometric bead array in cell lysate using MP50099-1

    Cytometric bead array in cell lysate using MP50099-1

    Cytometric bead array in cell lysate using MP50099-1, TFEB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68632-1-PBS. Detection antibody: 68632-2-PBS. Cell lysate: Raji，HepG2，HEK293.

  • WB analysis using 68632-1-Ig (same clone as 68632-1-PBS)

    WB analysis using 68632-1-Ig (same clone as 68632-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68632-1-Ig (TFEB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68632-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50099-1

    Cytometric bead array standard curve of MP50099-1

    Cytometric bead array standard curve of MP50099-1, TFEB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68632-1-PBS. Detection antibody: 68632-2-PBS. Standard:Ag24850. Range: 0.195-50 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy TFEB Ready-To-Use IHC Kit. TFEB primary antibody included.

产品信息

68632-1-PBS targets TFEB as part of a matched antibody pair:

MP50099-1: 68632-1-PBS capture and 68632-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen TFEB fusion protein Ag24850 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name transcription factor EB
Synonyms TCFEB, Class E basic helix-loop-helix protein 35, bHLHe35, AlphaTFEB, Alpha TFEB
Calculated Molecular Weight 476 aa, 53 kDa
Observed Molecular Weight53, 65-70 kDa
GenBank Accession NumberBC032448
Gene Symbol TFEB
Gene ID (NCBI) 7942
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19484
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calcualted molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).