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验证数据展示

  • WB analysis using 84675-5-RR

    WB analysis using 84675-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84675-5-RR (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84675-5-RR

    Affinity and Kinetic Characterization of 84675-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84675-5-RR against Human TFEB were performed. The affinity constant is 7.61 nM.

TFEB Recombinant antibody

TFEB Recombinant Antibody for WB, ELISA
Cat No. 84675-5-RR

产品说明书

CloneNo. 242093D11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

TCFEB, Class E basic helix-loop-helix protein 35, bHLHe35, AlphaTFEB, Alpha TFEB

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, RAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy TFEB Ready-To-Use IHC Kit. TFEB primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84675-5-RR targets TFEB in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 TFEB fusion protein Ag4194 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transcription factor EB
别名 TCFEB, Class E basic helix-loop-helix protein 35, bHLHe35, AlphaTFEB, Alpha TFEB
计算分子量 476 aa, 53 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC032448
基因名称 TFEB
Gene ID (NCBI) 7942
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP19484
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calculated molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).

实验方案

Product Specific Protocols
WB protocol for TFEB antibody 84675-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols