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验证数据展示

  • WB analysis of mouse testis using 13547-1-AP

    WB analysis of mouse testis using 13547-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13547-1-AP (TFEC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 13547-1-AP

    IP experiment of mouse testis using 13547-1-AP

    IP result of anti-TFEC (IP:13547-1-AP, 4ug; Detection:13547-1-AP 1:500) with mouse testis tissue lysate 4000ug.

TFEC Polyclonal antibody

TFEC Polyclonal Antibody for WB, IP, ELISA
Cat No. 13547-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

bHLHe34, hTFEC L, TCFEC, TFE C, TFEC, TFECL, transcription factor EC, Transcription factor EC like

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, COLO 320 cells
Positive IP detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13547-1-AP targets TFEC in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TFEC fusion protein Ag4061 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transcription factor EC
别名 bHLHe34, hTFEC L, TCFEC, TFE C, TFEC, TFECL, transcription factor EC, Transcription factor EC like
计算分子量 347 aa, 39 kDa
观测分子量35-40 kDa, 65 kDa
GenBank蛋白编号BC029891
基因名称 TFEC
Gene ID (NCBI) 22797
RRIDAB_2199629
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14948
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The DNA-binding factor TFE3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. TFEC shares a bHLH/LZ structure with TFE3 and a closely related protein microphthalmia-associated transcription factor (MITF), which is critically involved in melanocyte differentiation. The expression of TFEC is mainly in fibroblasts, myoblasts, chondrosarcoma cells and myeloma cells. 50kDa TFEC isoforms are produced by alternative splicing (PMID: 29303964, PMID: 11467950).

实验方案

Product Specific Protocols
WB protocol for TFEC antibody 13547-1-APDownload protocol
IP protocol for TFEC antibody 13547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols