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TFEC Polyclonal antibody

TFEC Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29324-1-AP

Print datasheet

Synonyms

bHLHe34, hTFEC L, TCFEC, TFE C, TFEC, TFECL, transcription factor EC, Transcription factor EC like

验证数据展示

  • WB analysis using 29324-1-AP

    WB analysis using 29324-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29324-1-AP (TFEC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29324-1-AP

    WB analysis using 29324-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29324-1-AP (TFEC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, mouse testis tissue, rat testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29324-1-AP targets TFEC in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen TFEC fusion protein Ag30973 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transcription factor EC
Synonyms bHLHe34, hTFEC L, TCFEC, TFE C, TFEC, TFECL, transcription factor EC, Transcription factor EC like
Calculated Molecular Weight 347 aa, 39 kDa
Observed Molecular Weight37-50 kDa
GenBank Accession NumberBC029891
Gene Symbol TFEC
Gene ID (NCBI) 22797
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14948
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The DNA-binding factor TFE3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. TFEC shares a bHLH/LZ structure with TFE3 and a closely related protein microphthalmia-associated transcription factor (MITF), which is critically involved in melanocyte differentiation. The expression of TFEC is mainly in fibroblasts, myoblasts, chondrosarcoma cells and myeloma cells. 50kDa human TFEC isoforms are produced by alternative splicing (PMID: 29303964, PMID: 11467950).

实验方案

Product Specific Protocols
WB protocol for TFEC antibody 29324-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols